RNA interference: Mechanisms and Applications
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چکیده
منابع مشابه
Small interfering RNA; principles, applications and challenges--
Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...
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Background: RNA interference (RNAi) is the mechanism of gene silencing-mediated messenger RNA degradation by small interference RNA (siRNA), which becomes a powerful tool for in vivo research, especially in the areas of cancer. In this research, the potential use of an expression vector as a specific siRNA producing tool for silencing of Bcr-abl in K562 cell line has been investigated. Methods:...
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In time, we have become gradually more aware of the fact that RNAs play a vital role in regulation of gene expression. The discovery of RNA interference (RNAi) also called post transcriptional gene silencing (PTGS), gave a booster shot to the world of RNA and molecular biology, a set of reactions involving the sequence-specific degradation of targeted mRNA by short double-stranded RNA molecules...
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Small non-coding RNAs such as small interfering RNA (siRNA), microRNA (miRNA) and piwi-interacting RNA (piRNA) exist in almost all kingdoms of organisms and have recently emerged as master regulators of gene expression to affect a diverse range of important biological processes. They exert their functions largely through two related but opposing mechanisms: RNA interference (RNAi) mediated by s...
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تاریخ انتشار 2006